Magnetite Nanoparticles Different Sizes Effectiveness On Growth And Secondary Metabolites In Ginkgo Biloba L. Callus

This study investigated the effects of magnetite nanoparticles (MNPs) various sizes (10.77, 20.5, and 29.3 nm), different concentrations (0.5, 1, 2 ppm) on growth production active constituents in Ginkgo biloba L callus. The best medium for callus proliferation was MS supplemented with 1 ppm naphthalene acetic acid (NAA), Kinetin, 0.5 MNPs a size 10 nm, which gave maximum fresh weight percentage increase after two subcultures. accumulation ginkgolide A bilobalide were achieved when elicited by nm at ppm. also focused estimating phenolic compounds scavenging free radicals (antioxidant activity) their indirect effect production. Also, had clear number compounds, where values recorded (2 + 10.77 nm). major components quercetin, kaempferol, p-coumaric acid, rutin, caffeic acid. Moreover, highest antioxidant activity (DPPH) treated nm) reached 77.29 %. highlights importance from L. focusing relationship between concentration. Besides, to provide good permanent source substances medical industrial large scale lowest cost.